Integrating Extracellular Matrix into 3D Spheroid Culture
AMSBIO has announced the launch of the 3D Spheroid Culture BME Matrices and Cell Assays to address the growing need for more complete and physiologically predictive cancer culturing and invasion models.
There is growing evidence that tumor cell aggregates or spheroids provide a more representative model of tumors in vivo than can be achieved with monolayers.
Spheroids exhibit several relevant physiological traits:
- similar morphology
- formation of cell-cell bonds
- increased cell survival
- tumor dormancy
- hypoxic core.
Applying this model to in a 3D culture invasion assay provides a more physiological approach for assessing tumor invasion and providing a visual component that can be quantitated through image analysis.
The Cultrex® Spheroid range provides a 3D Culture Qualified 96-Well Spheroid Formation Plate alongside a specialized Spheroid Formation extracellular matrix to drive tight spheroid formation of cells. Upon completion of spheroid formation, the spheroid is embedded in an invasion matrix composed of basement membrane proteins. This matrix forms a hydrogel network on which invasive cells can travel.
Show Spheroid Invasion/Proliferation Flowchart
Cancer stem cell invasion; using spheroid invasion matrix
- Courtesy of Walter Tinganelli, Biophysics Dept, Helmholtzzentrum für Schwerionenforschung GmbH (GSI)
Following spheroid formation, invasion modulating agents can be applied to the system to evaluate the impact on cell response. Cell invasion is visualized microscopically and can be quantitated through image analysis software.