AMSBIO now offers a new basal medium formulated for maximum growth and survival of proliferative neural cells such as human iPSC-derived neural stem cells or rat primary oligodendrocyte precursors.
NeuralX combined with GS22 to form a complete medium, demonstrate
superior performance in supporting the growth and survival of proliferating neural stem cells in culture.
- Improves long-term viability of proliferative neural cells
- Supports more efficient and higher yields of oligodendrocyte precursors
- Serum-free, animal-free for minimal risk of introducing adventitious agents into the cell
Supports Healthy Neural Stem Cells in Culture:
Figure 1. NeuralX Neural Stem Cell Medium and GS22 Neural Supplement support healthy growth of proliferating human iPSC-derived neural stem cells in culture over
multiple passages. HIP Human iPSC-derived pluripotent neural stem cells (BC1 line) were maintained in NeuralX NSC Medium and GS22 supplement for 4 days before passaging and continuing culture for another 4 days.
Optimal Differentiation into Healthy, Mature Oligodendrocytes:
Figure 2. Rat primary oligodendrocyte precursors exhibit excellent growth, survival and health when grown in NeuralX NSC Medium
and GS22 Neural Supplement.
After rat GRPs were grown for 3 passages in NeuralX NSC Medium with GS22 supplement, antibody labeling indicated the cells were still primarily undifferentiated GRPs, as indicated by labeling with A2B5 (green) and DAPI (blue).
Supports Growth &Long-term Survival in Oligodendrocyte Precursors :
Figure 3. Rat primary oligodendrocyte precursors differentiate into healthy, mature oligodendrocytes. After growing rat GRPs in in NeuralX
NSC Medium with GS22 Supplement for 3 passages, cells were switched to NeuralQ Basal Medium supplemented with GS21 Neural Supplement for 2 passages.
Antibody labeling revealed a significant number of mature, differentiated
oligodendrocytes, as indicated by labeling with GalC (green, left panel) and MBP (green, right panel). Total cells are shown using DAPI (blue, both panels