SoluLyse Protein Extraction Reagents
are optimized for the most efficient extraction of soluble proteins from bacterial
- Higher yields of soluble proteins
- Retention of more protein activity
- No interference with affinity purification resins
- Low viscosity without enzyme or sonication
- Simple 1-step protocol
- Compatible with protease inhibitors, salts, chelating agents & reducing agents
The SoluLyse Protein Extraction Reagent is a proprietary formulation of nonionic detergents that is optimized for the most efficient extraction of soluble proteins from bacterial cells. It allows perforation of bacterial cell wall without denaturing proteins, and there is no need for secondary treatment such as sonication or freeze-thaw.
Figure 1.Side-by-side comparisons between SoluLyse and another leading commercial lysis reagent showed that SoluLyse yields about 10-fold more soluble proteins. In addition, SoluLyse does not compromise protein activities in cell lysates.
The SoluLyse reagent is easy to use. Simply add it to a bacterial pellet and gently mix for 10 minutes. The cells will be disrupted and soluble proteins released. Insoluble cell debris can then be removed by centrifugation. SoluLyse is also useful for the preparation of high-purity inclusion bodies when the protein of interest is insoluble.
The SoluLyse Reagent is tested to be compatible with all popular purification methods, such as Ni and glutathione resins for purifying poly-His and GST-tagged proteins. The unique, mild formulation of SoluLyse offers minimized interference with the binding and elution processes and ensures superior purification results. In addition, the reagent is compatible with a variety of additives such as protease inhibitors and reducing agents and may be readily dialyzed if needed.