PD-L1 / TCR Activator Mammalian Expression Kit
Product Code
60610
$0.00
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The recombinant expression vectors are designed to express human engineered T cell receptor (TCR) activator and human PD-L1 (GenBank Accession #NM_014143) in mammalian cells. The transfected cells can be used in conjunction with PD-1/NFAT Reporter/Jurkat T cells ( #60535) to study the interactions of PD-1 with PD-L1 ligand in a cellular context and screen for modulators of this signaling pathway.In this assay|PD-1/NFAT Reporter/Jurkat T cells are used as effector cells; HEK293 cells overexpressing PD-L1 (or PD-L2) and an engineered T cell receptor (TCR) activator are used as target cells. When these two cells are co-cultivated|TCR complexes on effector cells are activated by TCR activator on target cells|resulting in expression of the NFAT luciferase reporter. However|PD1 and PD-L1 (or PD-L2) ligation prevents TCR activation and suppresses the NFAT-responsive luciferase activity. This inhibition can be specifically reversed by anti-PD1 or anti-PD-L1 antibodies. PD1/PD-L1 neutralizing antibodies block PD1:PD-L1 interaction and promote T cell activation|resulting in reactivation of the NFAT responsive luciferase reporter.
SKU | 60610 |
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Size | 500 reactions |
Alternate SKU | AMS.60610 |
Application | • Screen for activators or inhibitors of PD-1 signaling in a cellular context • Characterize the biological activity of PD-1 and its interactions with ligands |
Gene synonyms | Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1 |
Storage | Stable at least 6 months from date of receipt# when stored as directed. |
Shipping Temp | -20C |
Supplier Name | BPS |
Research Areas | Immunology |