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[UK] +44 (0) 1235 828200 ~ [CH] +41 (0) 91 604 55 22 ~ [DE] +49 (0) 69 77 90 99 ~ [US] +1 800 987 0985

Baculovirus Protein Expression

QUALITY YOU CAN TRUST
  • High level expression system
  • Competitive price
THE SERVICE AMSBIO PROVIDE
SCHEMATIC REPRESENTATION OF THE SERVICE AMSBIO PROVIDE

PHASE 1 :
  • Amplification/isolation of the gene of interest out of a customer-supplied vector and subcloning it into a transfer vector with Histag.
  • Sequence confirmation of insert.
  • Transformation of E. coli with baculoviral DNA and gene of interest.
  • Culture of E. coli and purification using mini-prep to produce bacmid (recombinant viral DNA).
  • Transfection of sf9 insect cells with bacmid.
  • Plaque purification and viral titer assay.
  • Small-scale culture to test expression level.
  • Estimated time: 4-6 weeks



PHASE 2 :
  • Virus stock preparation.
  • Large-scale culture and infection of insect cells for protein expression.
  • Purification from 1 litre of infected insect cell using affinity column of Ni-NTA or other column.
  • Estimated time: 2-4 weeks.



Products:
  • 5 µg transfer vector.
  • bacmid.
  • recombinant virus.
  • protein.
  • COA.

 baculovirus-protein-draw
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