Cellaria Cancer Cell Models and Media
AMSBIO now provides Cellaria patient specific cancer cell models and culture media that offer a simple method of derivation and long term culture of primary cells from patient tumors.
- Cells reflect morphology and histology of original tumor
- Simple media based expansion protocol
- Achieve upto 15 population doublings
- Stable genotype and growth rate
Once isolated from tumors cancer cell lines do not readily proliferate in culture, resulting in an extremely low success rate and as little as 0.7% of
patient tumors leading to cell lines that can be used as cancer cell model1. In addition tumor cell lines produced by traditional methods fail to
accurately reflect the characteristics present in tumors.
With the recognition that improvement in cancer treatment requires a focus on personalised medicines, these traditional and widely used cell lines have limited utility for predicting which drug protocol might be most effective for individual patients.
In response to a lack of reliable models AMSBIO now offers unique cell models and culture media technology that preserves the morphology, histology, and genomic profiles of cancer cells when compared to the original tumor.
Figure 1. Cellaria cell models are stable over time. At 20, 40 and up to 150 doublings the SNP prfile remains over 95%
identical to the original tumor profile in most models.
Cellaria Cancer Cell Models
Isolated from patient tumors AMSBIO offers Cellaria cancer cell models that unlike traditional methods for cell line generation are derived without any genetic manipulation.
Qualified by SNP analysis through 150 passages these cancer cell models do not exhibit large scale cellular adaptation in vitro and the associated genetic drift. As a result these cell models more accurately
represent the original tumor tissue and demonstrate predictable growth rates and stable proteomic expression.
Figure 2. The histopathology of the patient tumors (A and B) are recapitulated in the xenografts (C and D). The xenografts created with ATCC cell lines
(E and F) are not reflective of the patient tumor.
Cellaria cell models are stable and show high concordance to the original tumor genotype through 150 passages via SNP analysis.
They also exhibit predictable growth rates and stable proteomic expression. These models are fully consented, fully documented, and subject to extensive quality control.
Cellaria Cell Culture Media
Renaissance Media (RETM) Cancer Cell Media
RETM allows primary tumor cell derivation and maintenance of cancer cells.
- No feeder cells or cell genetic manipulation required
- Up to 95% success rate in establishing long term tumor cell culture
- One media supports multiple tumor types
- Primary tumor cells stable past 15 population doublings
- Stable genotype and growth rate for predictable cell expansion
- Cells reflects morphology and histology of original tumor
- Stable proteomic expression
- Low serum concentrations (2%-8%) are utilized
Specifically formulated for extended in vitro propagation, RETM media utilizes a simple, feeder-free protocol for multiple solid tumor types including breast, lung, colon, ovarian, and prostate. RETM produces cultures with
less variability and more reproducible results. Researchers have achieved over 95% success rate in establishing long-term culture of ovarian tumors, a success rate many times higher than in tumors cultured in traditional media, such as RPMI and MCDB.
WIT Normal Primary Cell Culture Media
WIT media is optimized for the expansion of normal human mammary epithelial cells and human fallopian tissue. These completely defined, serum-free media, support expansion over 20 passages without the growth arrest seen in other normal primary culture media.
WIT has been used for a broad range of applications
- Simplified long term culture of normal primary cells
- Cells exhibit a more luminal pattern compare to traditional media
1. Amadori, D. et al. Establishment and characterization of a new cell line from primary human breast carcinoma. Breast Cancer Res. Treat. 28, 251–260(1993).