Cellaria cancer cell lines and media
Cellaria are cancer cell line models and culture media for the most difficult to culture tumor types where no models may exist. Every model begins with an original patient tumor. Proprietary technology and methods are used, in order to preserve the diverse cell populations of patient tumors in stable, long-term cell models which are fully character¬ized and compared to the original tumor. Characterization of each cell model includes assessment of morphology, histology, and genomic profiles.
Fig 1. The histopathology of the patient tumors (A and B) are recapitulated in the AMSBIO Cellaria xenografts (C and D). The xenografts created with ATCC cell lines (E and F) are not reflective of the patient tumor.
Cellaria cancer cell models
AMSBIO offers a range of Cellaria cancer cell lines/models. Every Cellaria cell model is directly derived from patient tumors without any genetic manipulation and provides the fidelity of primary cells with long-term reproducibility and scalability. Unlike traditional methodologies for cell line creation, Cellaria’s cancer models eliminates large scale cellular adaptation in culture and genetic drift.
AMSBIO Cellaria’s cell models are stable and show high concordance to the original tumor genotype through 150 passages via SNP analysis. They also exhibit predictable growth rates and stable proteomic expression. These models are fully consented, fully documented, and subject to extensive quality control.
Cellaria Cancer cell models
Cellaria cell models are screened for all major categories of cell contaminants, including:
- Microbial contaminants - sterility testing
- Viruses - human pathogens screen
- Cell line cross-contamination: interspecies & intraspecies - STR profiling
Lot specific growth rate and protocol is provided for every customer. Our QC process ensures stable growth rate for each lot.
Cellaria cell culture media
AMSBIO also has a range of Cellaria cell culture media. Such as Renaissance Essential Tumor Medium (RETM) and WIT culture media for the primary normal cell culture.
Renaissance Essential Tumor Medium (RETM)
Is a cell model derivation and maintenance medium for successful cultivation of cancer cells. Specifically formulated for extended in vitro propagation, Renaissance media utilizes a simple, feeder-free protocol for multiple solid tumor types including breast, lung, colon, ovarian, and prostate. RETM produces cultures with less variability and more reproducible results. Researchers have achieved over 95% success rate in establishing long-term culture of ovarian tumors, a success rate many times higher than in tumors cultured in traditional media, such as RPMI and MCDB.
WIT culture media for the primary normal cell culture
Is a line of media optimized for the expansion of normal human mammary epithelial cells and human fallopian tissue. These are completely defined, serum-free media. WIT media support expansion over 20 passages without the growth arrest seen in other normal primary culture media. WIT has been used for a broad range of applications by scientists including growth of lymphoma and mouse tumor tissue.