Product Search:
x Select your country:

WorldMap

[UK] +44 (0) 1235 828200 ~ [CH] +41 (0) 91 604 55 22 ~ [DE] +49 (0) 69 77 90 99 ~ [US] +1 800 987 0985

Monoclonal Antibody

  • Free peptide design.
  • Immunization of 5 Balb/c mice.
  • Fusion of spleen cells with SP2/0 myeloma cells.
  • ELISA screening for up to 3000 clones.

  • Free Western blot screening for protein antigen.
  • Guaranteed IgG isotype.
  • Progess tracking.
  • ELISA positive guaranteed or your money back.
THE SERVICE PROVIDED BY AMSBIO

Western blot analysis using LIN28 mouse mAB hIgGFc-LIN28 transfect ChO-K1, and 4 NTERA-2 cell extract.

Confocal immunofluorescent analysis of PC3 cells using GSTP1 mouse mAb (green).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue, showing cytoplasmic location using vimentin mouse mAb.

PHASE 1 : IMMUNIZATION AND ELISA EVALUTATION
  • Immunization and boosts of 5 Balb/c mice with standard immunization protocol.
  • ELISA evaluation of titer prior to selection for fusion.
  • Approximately 3-4 mg protein immunogen or 3 mg conjugated peptide immunogen and 1 mg free peptide are required.
  • Estimated time: 8~10 weeks.



PHASE 2 : HYBRIDOMA FUSION AND SCREENING OF SPECIFIC CLONES
  • Once an acceptable titer is obtained, hybridoma fusion will be done using splenocytes from the mouse with the best titer and myeloma cells.
  • Supernatants from the growing hybridoma wells will be screened by ELISA. Supernatant from 10 different ELISA positive clones will be sent to customer's lab for evaluation.
  • Estimated time: 4~5 weeks.



PHASE 3 : SUBCLONING
  • Two of these ten clones selected by the customer will be subcloned by limiting dilution, isotyped, and the cell line will be shipped to the customer.
  • Estimated time: 6~8 weeks.



Note:Production and purification services avaiable. Please enquire.
amsbio - custom services icon Email your Lab Manager/Colleague this page url:
Your Email Address: Colleague's Email Address:
AMS Biotechnology (Europe) Limited, 184 Park Drive, Milton Park, Abingdon OX14 4SE, UK.
Telephone: (UK) +44 1235 828 200 (CH) +41 91 604 55 22 (DE) +49 69 77 90 99, VAT No. GB 502 2372 95
Usage of this web site is governed by our privacy policy, our web site Terms and Conditions,
our use of the web site Terms and Conditions and our Terms of Sale
Reg. in England and Wales no. 2117791. ISO 9001 registered firm. ©2013