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[UK] +44 (0) 1235 828200 ~ [CH] +41 (0) 91 604 55 22 ~ [DE] +49 (0) 69 77 90 99 ~ [US] +1 800 987 0985

Immunohistochemstry Research





We offer immunostaining services to localize specific antigens in any paraffin and frozen tissue section from Human and mammalian species provided by customer or by us.

Single Immunohistochemistry:

  • Method of detection: immunofluorescence, alkaline phosphatase, immunoperoxidase

Double Immunohistochemistry:
  • Double immunohistochemistry (IHC) : immunolocalization of 2 or more antigens in the same tissue slide
  • Method of detection: immunofluorescence
  • Double stains can be mounted permanently
  • Obtain more information from each slide and reduce turnaround-time compared to single staining
  • It is possible to assess the topographic relationship of the targets
  • In addition, allows the combination of in situ hybridization (ISH) and IHC, giving information at protein level and DNA/mRNA level


Immunostaining

Fig. Double immunostaining in Langerhans Islets


THE SERVICE PROVIDED BY AMSBIO
Services Description
Immunohistochemistry
  • Optimization of tissue fixation process : select fixative agent and determine incubation time
  • Optimization of antigen retrieval conditions: use of detergent, enzymatic or microwave retrieval
  • Titration of the primary antibody concentration and adjustment of the experimental conditions of the immunostaining
  • Interpretation of results
  • Image and quantitative analysis of immunostaining if requested
  • Deliver report and high quality digital images (prints is an option)


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AMS Biotechnology (Europe) Limited, 184 Park Drive, Milton Park, Abingdon OX14 4SE, UK.
Telephone: (UK) +44 1235 828 200 (CH) +41 91 604 55 22 (DE) +49 69 77 90 99, VAT No. GB 502 2372 95
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