Product Search:
x Select your country:

WorldMap

[UK] +44 (0) 1235 828200 ~ [CH] +41 (0) 91 604 55 22 ~ [DE] +49 (0) 69 77 90 99 ~ [US] +1 800 987 0985

Mammalian Protein Expression

ADDITIONAL SERVICES
  • High level expression system.
  • Competitive price.
  • Human cell line origin with full glycosylation.
  • Scale up to 500 litres.
  • Tag removal as requested.
THE SERVICE AMSBIO PROVIDE
SCHEMATIC REPRESENTATION OF THE SERVICE AMSBIO PROVIDE
PHASE 1 : Cloning of your gene of interest into a vector with His-tag or IgG-Fc tag.

  • Sequencing confirmation of insert.
  • Small scale (5ml) transfection to test the expression level and optimization.
  • Estimated time: 3-4 weeks



  • PHASE 2 : Large-scale transfection (500ml) of recombinant vector into HEK293 and protein expression, evaluation and optimization.

  • One litre of bacterial culture will be induced with IPTG and harvested for protein purification.
  • Protien purification from 500ml of HEK293 culture using affinity column of Ni-NTA or protein A (no tag cleavage service provided).
  • The yield ranks from 3-25mg per 500ml with an average of 5mg.
  • Estimated time: 2-3 weeks.


  • Deliverables from phase 2:

    Protein and report including sequence confirmation, Western blot and SDS-PAGE result and design form.

     bacterial-expression-draw
    amsbio - custom services icon Email your Lab Manager/Colleague this page url:
    Your Email Address: Colleague's Email Address:
    AMS Biotechnology (Europe) Limited, 184 Park Drive, Milton Park, Abingdon OX14 4SE, UK.
    Telephone: (UK) +44 1235 828 200 (CH) +41 91 604 55 22 (DE) +49 69 77 90 99, VAT No. GB 502 2372 95
    Usage of this web site is governed by our privacy policy, our web site Terms and Conditions,
    our use of the web site Terms and Conditions and our Terms of Sale
    Reg. in England and Wales no. 2117791. ISO 9001 registered firm. ©2013