Pre-made human and mouse miRNA expression lentivirus
Human or mouse microRNA precursors and their native context sequences (upstream and downstream flanking genomic sequences) were PCR amplified, and cloned into a pLenti-TetCMV(GFP-Stop-3UTR/miRNA)-Rsv(Puro) lentivector. The GFP and pre-miRNA are co-transcribed under the same promoter, the optional inducible CMV promoter. The GFP provides a convenient indicator for miRNA expression levels. A puromycin antibiotic selection marker provides the selection method for long term stable expression. See the scheme below for the core vector structure.
Sequence verified miRNA expression lentivector is co-transfected with lentiviral packaging plasmids (Cat#: HT-pack) into lentivirus production cell line (Cat#: TLV-C). The VSV-G pseudotyped lentivirus is packaged in DMEM medium with 10% serum following virus production protocols. Virus is filtered through 0.45um filter, and provided as 200ul aliquot.
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- Each order will contain two pre-made miRNA lentiviruses
- 0.5 ml of pre-made specific miRNA lentivirus (the desired specific miRNA),
- 0.5 ml of negative control miRNA lentivirus
- miRNA expression product manual
- Virus titer: 1x10e7 IFU/ml
Product Lists and ordering:
Human miRNA expression lentivirus product list
Mouse miRNA expression lentivirus product list
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