Poly-D-Lysine, a highly positively charged synthetic amino acid chain, is commonly used as a coating agent to promote cell adhesion in culture on TCT plastic or glass surfaces. Moreover, poly-D-Lysine is resistant to enzymatic degradation and promotes the proliferation and differentiation of a variety of neuronal cell lines. This solution is provided ready to use at 0.01% and contains polymers in the 70,000-150,000 kDa range.
The wells with the 0.01% Cultrex® poly-D-Lysine sample must exhibit cell attachment of equal to or greater than 10% whereas uncoated wells must have less than 5% attachment to pass quality control. l