Type I collagen is the major structural component of extracellular matrices found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37°C. This phenomenon can be exploited to promote cell attachment, proliferation, differentiation, migration, and tissue morphogenesis during development.
Gelled rat collagen I should support a formation of network-like structures of SVEC4-10 cells compared to the cells adherent to the uncoated plastic surfaces only. The wells with the 20µg/ml Cultrex® Collagen I sample must exhibit cell attachment of equal to or greater than 40% whereas uncoated wells must have less than 10% attachment to pass quality control