shRNA lentivirus construction:
You choose the markers you want (see vector map scheme). We construct the lentiviral inducible*
shRNA clones and generate 0.5 ml (or more if requested) of ready-to-use shRNA lentivirus.
If you provide the knockdown sequence, we simply make the shRNA DNA oligo-duplex and clone it into the shRNA vector.
If you can’t already provide an efficient knockdown sequence, we will design a set of 3 shRNA constructs.
We guarantee that at least one of these 3 shRNA constructs shows a knockdown of ≥75% of the target RNA and we provide a
free negative control shRNA lentivirus
Why use AMSBIO's lentivirus services :
- Safe-to-use (self-inactivating) lentiviral particles can deliver your gene into a wide range of cell lines including non-dividing, primary or stem cells
- Engineered in-house lentiviral vector for highly efficient gene integration into cell genome.
- Our lentivectors have the strongest promoter for the highest protein expression in mammalian cells.
- You can choose to have an inducible* or constitutive expression.
- High titer of lentivirus that is monitored by a fluorescent protein (not fused with your target).
- Our experts with years of experience in lentiviral cloning and expression.
- Fast turnaround time.
- The best price and the best quality in its class.
Some of AMSBIO others services:
High titer ready-to-use expression Lentivirus for your targets (around 107 TU/ml).
Generation of stable cell lines in almost any mammalian cell line.
Protein expression in mammalian suspension cells using lentiviral transduction.
Affinity purification (His-tag) of recombinant proteins expressed in mammalian cells.
Additional information on our inducible H1 promoter:
The transcription of shRNA is driven by our optional inducible human H1 RNA polymerase III promoter.
Without any intervention, our H1 promoter constitutively expresses high levels of shRNA.
To take advantage of the promoter’s inducible properties, the repressor protein TetR must be present.
The expression of TetR can be achieved by using our
pre-made Tet-repressor lentiviral particles
or by using our
stable Tet-repressor cell line. In the presence of TetR, the H1 driven shRNA transcription
is blocked and expression can be induced by addition of tetracycline.
Please note that although our lentiviral vectors contain all
necessary bio-safety features, work with lentiviral particles should be carried out
Biological Safety Level 2 (BL2)
or higher. Please conduct a thorough risk assessment for
your project and contact your health and safety facilities for local guidelines and regulations.