Efficient extraction of proteins from mammalian cells
- High yields of non-denatured proteins
- Optimized protocols for both adherent and suspension cells
- Simple and rapid protocols
- Compatible with downstream applications
The SoluLyse-M Mammalian Protein Extraction Reagent is a novel nonionic detergent designed for efficient whole-cell protein extraction from cultured mammalian cells. It gently and rapidly dissolves cell membranes at low concentrations without denaturing proteins.
SoluLyse-M Mammalian Protein Extraction Reagent provides a highly efficient yet gentle method for extracting proteins from mammalian cells. It extracts cytoplasmic and nuclear protein from cultured cell lines using a proprietary non-ionic detergent in 25mM Tris, pH 7.4 and 250mM Sucrose. Lysis with SoluLyse-M reagent is so efficient that adherent cells do not need to be scraped from the culture dish or multi-well plates. Total protein can be recovered by simply adding an appropriate amount of SoluLyse-M reagent into the container and shaking for 10 minutes. This is especially important when the cells are grown in 96-wll or 24-well plates.
SoluLyse-M Mammalian Protein Extraction Reagent is compatible with many different applications, such as reporter assays, immunoassays and protein purification. The reagent is easily dialyzable and compatible with protein assays such as Western blotting, Coomassie Blue, Bradford and the BCA Protein Assays. In addition, the reagent is compatible with a variety of additives such as protease inhibitors and reducing agents and may be readily dialyzed if needed.
Compatibility with Protein Purification.
SoluLyse-M reagent was used to lyse CHO-K1 cells expressing 6xHis-LacZ. The lysate was purified with Ni-NTA resin. Western blotting was performed using anti-His antibody.
- SoluLyse-M Protein Extraction Reagent is supplied in 1X solution in 25mM Tris, pH 7.4 and 250mM Sucrose.
- Store at room temperature or +4o
C upon receipt. Stable for 2 years when stored properly.