Features

  • Produced using GMP bovine type I collagen, and resembles natural collagen fiber structures
  • Can be resuspended in any aqueous environment, including acidic environments (pH≥2)
  • Compatible with all common bioinks, including GelMA, alginate, gelatin and agarose
  • Validated with a variety of cell lines including fibroblasts, myoblasts, epithelial cells, neural cells and primary cells such as bone marrow derived MSCs
  • Available with fluorescent tag

Benefits

  •  Increases mechanical strength and modulus of hydrogels in extension and compression
  • Improves shape retention/durability for at least 28 days in bioprinted cellular constructs
  • Acts as a physiologically relevant site for cell attachment within the constructs
  • Improves shape fidelity without compromising bioink viscosity/printability
  • Aligns with printhead flow, avoiding clogging for 3D printing applications.

Product Information Table

NameDatasheetPacksize Order
CollaFibR Additive (for Bioinks and Hydrogels). No FITC/ short fibers.25 mg View
CollaFibR Additive for Bioinks and Hydrogels. No FITC/ short fibers.10 mg View
Fluorescent CollaFibR Additive for Bioinks and Hydrogels. FITC Tag/ short fibers25 mg View
Fluorescent micro-CollaFibR (TM) Additive for Bioinks and Hydrogels. FITC Tag, short fibers10 mg View

Mechanical performance of Bioink with added μCollaFibR™

2.5 mg/mL of μCollaFibR™ improves the extensional strength of 7.5%wt GelMA constructs by 88%. (B) 1.25 mg/mL of μCollaFibR™ improves the compressive strength of 5%wt GelMA constructs by 116%.

Improved Shape Retention with added μCollaFibR™

Cellular constructs with (A) μCollaFibR™ have over 90% shape retention after 28 days in culture, compared to (B) 25% shape loss after only 6 days in culture in the control. The mold diameter is 11 mm.

Improved Cell Functionality with added μCollaFibR™

In bioinert constructs, μCollaFibR™ provides a physiologically relevant site for cell attachment, improving cell functionality. Scale bar = 100 μm.

μCollaFibR™ as microcarrier for Bioreactors

μCollaFibR™ can provide a physiologically relevant attachment site for cell expansion in bioreactors, which can increase yield of cells and/or biologics of interest.

μCollaFibR™ eliminates the need for trypsin in cell recovery, preserving the natural protein structure of cells and potentially reducing costs and time required in cell recovery.

Collagenase Degradation of CollaFibR™ for Cell Recovery

Our Collagenase NB and Neutral Protease NB Enzymes are the perfect choice for degrading CollaFibR™ for easy cell extraction.

μCollaFibR™ Additive Frequently Asked Questions

What is the diameter of μCollaFibR™?

μCollaFibR™ is 1-2 micrometers in diameter in the dry state and 8-10 micrometers in diameter in the wet state.

What is the length of μCollaFibR™?

The average length of μCollaFibR™ is 44 ± 13 microns.

What bioinks are compatible with μCollaFibR™?

Most common bioinks, including GelMA, alginate, agarose, and gelatin are compatible with μCollaFibR™.

Which solvents are compatible with μCollaFibR™?

μCollaFibR™ can be dispersed in water, buffered salt solutions e.g. PBMS, weak acids (e.g. 10 mM HCL, 20 mM AA) and most off-the-shelf bioinks including gelMA, alginate, agarose, and gelatin.

What is the stability of μCollaFibR™ in acidic conditions?

μCollaFibR™ is stable in 10 mM HCL for up to 6 months.

What is the recommended storage temperature for μCollaFibR™?

μCollaFibR™ is shipped at RT and once received, please store between 2-8°C for short term storage (up to 3 months) and -20°C for longer term storage.

What is the maximum working temperature for μCollaFibR™?

The maximum temperature is below 60°C.

What sterilisation method is recommended for μCollaFibR™?

μCollaFibR™ is supplied in sterile packaging and is ready for use. Do not autoclave the product as it is not stable at temperatures above 60°C. Do not filter sterilise the product as the fibers are too large to pass through a filter.

What cell types are compatible with μCollaFibR™?

μCollaFibR™ has been validated with a variety of cell lines including fibroblasts, myoblasts, epithelial cells, neural cells and primary cells such as bone marrow derived MSCs.

Can I buy fluorescent μCollaFibR™?

Yes, μCollaFibR™ can be purchased in both fluorescent and non-fluorescent formats.

What is the source of Collagen used in μCollaFibR™?

Type I collagen sourced from bovine hide (skin) manufactured in a GMP facility.