AAV Reference Standards
A safe strategy for gene therapy.
Adeno-associated virus (AAV) is a virus that can be engineered to transport DNA of interest to target cells, without any viral genes. There has been a revival in gene therapy efforts, particularly in research and clinical applications, at least partially powered by the progress in understanding of AAV. The potential of recombinant AAV particles is sky-high and is proven to be one of the current safest strategies for gene therapy.
Arguably the greatest challenge in AAV production is that AAV packages a significant number of viral particles without gene payloads. Even after purification procedures, there will still be empty capsids. Therefore, a well-characterized AAV standard with an accurate full/empty ratio is needed to distinguish the final vector production and accurately compare AAV success.
Benefits
- Multiple AAV serotypes available
- Accurate Full/Empty Ratio Quantification
- Extremely high percentage of empty capsids of reference materials – 85%-99.5% empty particles
- Very high percentage of full capsids – 55%-82% depending on the serotype.
- High virus concentration - >1x10^12 VP/mL
- Extensive analyses – Silver Staining, ELISA, TEM, Bioburden, Mycoplasma, and Endotoxin
- Reliably Quantified - Calibrated with ATCC Reference Standard Material
Name | Datasheet | Packsize | Order |
---|---|---|---|
AAV1 Reference Standards (Full Capsids) | 100 ul | View | |
AAV2 Reference Standards (Full Capsids) | 100 ul | View | |
AAV5 Reference Standards (Full Capsids) | 100 ul | View | |
AAV6 Reference Standards (Full Capsids) | 100 ul | View | |
AAV8 Reference Standards (Full Capsids) | 100 ul | View | |
AAV9 Reference Standards (Full Capsids) | 100 ul | View |
High quality and high purity empty capsid standards are used as reference materials in an extensive range of applications for example, qPCR, ELISA, HPLC etc.
AMSBIO provide extensively analyzed AAV reference materials with viral particle concentration and full/empty ratio. These are provided in the form of 5 serotypes: AAV1, AAV2, AAV5, AAV6, AAV9
Both full and empty capsids are available for many serotypes.
Our reference materials are well-characterized, including bioburden, endotoxin, mycoplasma testing, silver staining (Figure 1), ELISA, qPCR and transmission electron microscopy (TEM) (Figure 2) and are quantified based on the reference standard material from ATCC.
The stability of our reference material will be tested in an ongoing manner to ensure reliability.
Citations
Unveiling the secrets of adeno-associated virus: novel high-throughput approaches for the quantification of multiple serotypes.
Meierrieks, F., Kour, A., Pätz, M., Pflanz, K., Wolff, M. W., & Pickl, A. (2023). Unveiling the secrets of adeno-associated virus: novel high-throughput approaches for the quantification of multiple serotypes. Open Medicine Technology, 1, 101118.