AMV-RT High Spec Activity

AMV RT-XL is a superior grade of native reverse transcriptase isolated from Avian Myeloblastosis virus.  This enzyme synthesizes cDNA from single-stranded RNA.   Selected for very high specific activity|concentration and purity. Applications: cDNA Library production|First strand cDNA synthesis for cloning|RNA sequencing|RT-PCR|High-temperature cDNA Synthesis at up to 50ºC. Reagent supplied|with a protocol for cDNA synthesis:  AMV Reverse Transcriptase 10X Reaction Buffer (Catalog # ARB 45). At 1X concentration the magnesium ion concentration is only 5 mM. Under this condition|endogenous RNaseH activity is not active. Unit Definition:  One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37ºC using  poly(rA)-d(T)12-18 as template primer. Unit Assay Conditions: 0.5 mM [3H]-TTP|0.4 mM rAn.d(T)12-18|50 mM TrisHCl|pH 8.3|40 mM KCl|6.0 mM MgCl2|and 4.0 mM DTT.  AMV RT preparations are diluted in a dilute phosphate buffer and added to the pre-incubated reaction to give linear kinetics. 50% Glycerol based storage buffer. Storage Temperature: -70ºC

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