Cas9 Inducible (Tet-On) iPS Cell Pool

Product Code
78845
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The Cas9 Inducible (Tet-On) iPS Cell Pool expresses the Cas9 (Streptococcus pyogenes CRISPR associated protein 9) gene under the control of a tight TRE tetracycline-inducible promoter/ introduced into PBMC-derived human iPS cells via lentiviral transduction [Cas9 Lentivirus (inducible TET on)/ #78794]. Cas9 expression can be induced with doxycycline treatment/ allowing temporal control of its expression. Cas9 Inducible (Tet-On) iPS cells can be transduced with single-guide RNA (sgRNA) targeting gene(s) of interest to generate genetically engineered cell pools or cell lines.
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SKU 78845
Size 1 vial
Application • Study molecular pathways using temporally controlled induction of gene editing.• Generation of sgRNA-edited iPS cell pools.• Screening of pooled sgRNAs libraries to identify pluripotency/ differentiation/ and drug toxicity genetic modulators.
Gene synonyms iPSC
Storage Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt.
Shipping Temp -80C (dry ice)
Supplier Name BPS