Exosome Isolation
Here at AMSBIO, we have developed and optimized methods and tools for fast, scalable and reproducible Extracellular Vesicle (EV) purification, as well as addressing EV heterogeneity and high throughput solutions for biomarker discovery.
Although ultracentrifugation is still widely used, it tends to alter the vesicle shape and functionality.
It's time-consuming methodology and need for expensive equipment has meant that it is no longer considered the gold standard for EV isolation.
Our products however; offer fast, cost-effective solutions for all your EV isolation needs.
Tangential Flow Filters for EV concentration and purification
Tangential flow filtration (TFF) is a rapid and efficient method, usually used for separation and purification of biomolecules in industrial applications and can be successfully applied to isolate and separate extracellular vesicles. We provide three different typologies of filters suitable respectively for EV purification, EV concentration and EV size-based separation.
TFF-Simple: Filter for EV concentration and dialysis
TFF-Simple is a filter cartridge in hollow fibers made of polysulfone, which allows the particle concentration and the removal of small proteins and molecules from diluted matrices (cell conditioned media, urine, etc..), prior to the EV purification.
Applications
- Concentration of diluted fluid as cell media or urine prior to EV isolation
- Easy removal of small molecules and ions from the EV preparation
- EV dialysis and buffer exchange
- High efficiency of EV isolation if coupled with SEC columns
Advantages
- Washable
- Reusable multiple times. Easy to use
- Fast concentration of EV containing matrices
TFF-Micro: Filter for large EV separation and purification
TFF-Micro is a filter able to separate large microvesicles (MVs) by size, avoiding the separation by centrifugation at 10000g, which often causes the loss of part of small EVs. TFF-Micro retains vesicles larger than 150-200 nm, whereas small EVs and circulating molecules pass in the permeate. Retained MVs can be recovered with a syringe in PBS buffer, without additional purification steps.
Applications
- Separation, concentration and recovery of large EVs (>150 nm)
- Large EV isolation from cell media, biofluids, plant extracts
- Dialysis and desalting of large EVs
- Suitable for large EV isolation from 5 ml of fluid
Advantages
- Washable
- Reusable multiple times
- Sterile
- Fast separation of EVs bigger than 150 nm
Name | Datasheet | Packsize | Order |
---|---|---|---|
TFF-MV- tangential flow filter for large EV separation & recovery | 1 concentrator | View |
TFF-Exo: Fast EV purification
TFF-Exo is our next-generation filter which allows a rapid, reproducible and scalable purification of EVs, can be used on the lab bench for purifying small amount of samples (min 5 ml) or connected with a mechanical system for purifying larger volumes.
TFF-Exo is a filter cartridge made of polyethersulfone hollow fibers with pores of 50 nm size (cut off 300 kDa). The filter allows the purification of EVs and particles > 50 nm.
Applications
- Separation, purification and concentration of EVs from conditioned media, biofluids, plant extracts
- Buffer exchange and removal of unbound dye
- Depletion of FBS from bovine EVs
Advantages
- Washable
- Reusable multiple times
- Sterile
ExoSpin: Ultrafiltration concentrator
ExoSpin is a 2.5 ml non-stick ultrafiltration (UF) concentrator to enhance the recovery of extracellular vesicle (EVs) during the concentration or buffer exchange step. The ultrafiltration works in the opposite direction to the centrifugal force, providing higher particle recovery. The low protein binding membrane (polyether- sulphone) reduces the EV loss, compared to V-shape concentrators, and the reverse design of the EV-spinner ensures that the filter does not clog.
ExoSpin Products
Name | Datasheet | Packsize | Order |
---|---|---|---|
EV-Spinner: Ultrafiltration concentrator, MWCO 100 kDa, 24 pcs. | 24 concentrators | View |
ExoUltra: Size exclusion chromatography
Size Exclusion Chromatography (SEC) is an efficient method for isolating and purifying Extracellular Vesicles (EVs) from different fluids, not affecting the original shape and functionality of the vesicles. Our range of ExoUltra SEC columns allow for EV purification from small (100 µl) to large volumes (up to 20 ml) of fluids. EV purification with our ExoUltra columns takes approximately 15 minutes allowing integration into any laboratory workflow.
Characteristics
- New gel matrix for EV purity improvement
- Purification up to 20 ml volume of fluid
Advantages
- Easy and fast protocol (turnaround time approximately 15 minutes). Isolate EVs from small sample volumes
- Reusable up to 5 times
- Long term stability at 4°C
Applications
- Extracellular vesicles isolation from cell media, biofluids and plant extracts
- Purification of EVs from contaminants
- Dye excess removal post EV labelling process
Name | Datasheet | Packsize | Order |
---|---|---|---|
PURE-EVs Complete kit: Size exclusion chromatography column/ TFF-easy/ TFF-MV | - | 1 ma x i column + 2 tff concentrator | View |
PURE-EVs PLUS: Size exclusion chromatography columns & TFF concentrator | - | 5 columns + 1 TFF concentrator | View |
PURE-EVs: Size exclusion chromatography column | 10 columns | View | |
PURE-EVs: Size exclusion chromatography column | 5 columns | View | |
maxiPURE-EVs PLUS: Size exclusion chromatography column & TFF concentrator | - | 1 ma x i column + 1 tff concentrator | View |
maxiPURE-EVs: Size exclusion chromatography column | 3 columns | View | |
maxiPURE-EVs: Size exclusion chromatography column | 6 columns | View | |
miniPURE-EVs: Size exclusion chromatography column | 10 columns | View | |
miniPURE-EVs: Size exclusion chromatography column | 20 columns | View |
ExoUltra: Immunoaffinity isolation
Understanding and addressing extracellular vesicle heterogeneity has become essential for the EV field, especially because of the different roles that EVs have in pathological processes such as cancer, infection, and neurodegenerative diseases.
Our pre-coated ELISA immunoplates and our latex or magnetic immunobeads are perfect for the capture and enrichment of total or specific EV-subpopulations.
Applications
- Multiple profiling of EV markers from both a single sample or screening a large number of samples
- EV capture and quantification from human biofluids (plasma, serum, urine, saliva)
- Immunocaptured EVs are suitable for downstream processes such as nucleic acid extraction
Advantages
- Ready to use.
- Long term storage (24 months).
- No EV pre-purification required
- Small sample amounts required (100 μl per well).
- Flexibility and ease when designing a multiplexing assay.
- Open platform for customized coating solutions.
Name | Datasheet | Packsize | Order |
---|---|---|---|
Immunoplate for capture L1CAM positive exosomes | 1 plate | View | |
Immunoplate for capture CD63 positive Exosomes | 1 plate | View | |
Immunoplate for capture EphrinB positive exosomes | 1 plate | View | |
Immunoplate for capture PLP1 positive Exosomes | 1 plate | View | |
Immunoplate for capture TM9SF4 positive exosomes | 1 plate | View | |
Immunoplate for capture of CD9 positive Exosomes | 1 plate | View |
ExoPure: Chemical isolation
Our ExoPure is a chemical precipitation based, fast and convenient method of Extracellular Vesicle isolation from bio-fluids, cell culture supernatants and plant extracts.
Samples are incubated on ice with our ExoPure solution so that EVs will precipitate following centrifugation. The obtained pellet can be re-suspended in PBS 1X or deionized water. Our user-friendly protocol is time-saving and does not require expensive laboratory equipment. These isolated vesicles are suitable for isolation of EV associated nucleic acids.