RNArchive
Ambient storage and protection of RNA samples for decades
Stabilize RNA in solid or liquid form at room temperature
• Protection for years at room temperature
• Safe and secure shipping with no dry ice
• Undergo up to 5 rehydration cycles with no loss in RNA integrity
Features
RNArchive ensures:
- Stability under extreme conditions when dried
- Increased stability in liquid state for up to 100 hours at room temperature (21-25°C)
- Ability to go through up to 5 rehydration cycles with high RIN values
- Does not inhibit RT-qPCR or expression profiling
- Compatible with RNA purified using all standard kits
and protocols
Applications
Available in multiple formats, RNArchive provides low cost solutions for:
- Safe shipping and handling
- RNA biobanking
- High quality, post-storage RNA seq
Simple workflow
High levels of RNA protection come with a simple process. Just collect, dry and rehydrate your samples when required with molecular biology grade water. Your samples with be able to withstand 5 rehydration cycles.
Consistently high RIN scores after long-term storage
Shipping RNA on dry ice has become increasingly common. This has lead to a huge rise in expense, but also an increase in the loss and damage of samples on transit. Improper dry ice maintenance causes the thawing and degradation of samples. AMSBIO offers a convenient solution to eliminate dry ice, expensive packaging and overnight shipping.
As well as reducing costs RNArchive will provide peace of mind as the risk of sample thawing is totally negated. The robust protection at room temperature ensure the highest quality and safety of your RNA samples, allowing highly efficient subsequent research.
High quality RNA at a range of temperatures
RNA samples stored in the dry state on RNArchive show no degradation after the equivalent of four years of ambient temperature storage.
Product Information Table
Name | Datasheet | Packsize | Order |
---|---|---|---|
GenTegra-RNA dry Bulk, for 100 custom tubes or 100 automated samples | for 100 custom tubes or 100 automated samples | View | |
GenTegra-RNA 0.5ml Cluster Tubes, 96 Tubes per Rack, 1-Rack, No Barcode | 96 Tubes per Rack (1-Rack) | View | |
GenTegra-RNA 0.5ml Cluster Tubes, 96 Tubes per Rack, 10-Racks, Barcode | 96 Tubes per Rack (10-Racks) | View | |
GenTegra-RNA 0.5ml Cluster Tubes, 96 Tubes per Rack, 10-Racks, No Barcode | 96 Tubes per Rack (10-Racks) | View | |
GenTegra-RNA 0.5ml Screw Cap Tube, 100-Pack, supplied 4-envelopes of 25 tubes ea. | 100-Pack supplied 4-envelopes of 25 tubes ea. | View | |
GenTegra-RNA 0.5ml Screw Cap Tube, 25-Pack (Trial pack) | 25-Pack | View | |
GenTegra-RNA 0.5ml Screw Cap, Sample test kit 3-Pack, with protocol | Sample test kit 3-Pack | View | |
GenTegra-RNA 96-Well Plate, Barcode, 10-Pack | 10-Pack | View | |
GenTegra-RNA 96-Well Plate, Single, No Barcode (Trial plate) | Single | View |
FastDryers
FastDryers are a simple and convenient way to dry
biosamples following application to RNArchive (96-
well microplates, racks of 0.3 ml cluster tubes, and 0.5 ml
screw-cap tubes) and RNArchive (0.5 ml screw-cap tubes
and 0.3 ml cluster tubes),
Application Volume | Fast Dryer Drying Time | Biosafety Hood Drying Time |
---|---|---|
20µL | 10 hours | ~18 hours |
21-50µL | 16 hours | ~24 hours |
RNArchive Frequently Asked Questions
Option 1 - Following application to RNArchive use RNA in liquid form for up to 100 hrs at room temperature
(21-25°C) or on ice. RNArchive conveys additional stability to RNA in liquid form by inactivating trace RNase, simplifying sample handling. Following the 100 hour period, dry the sample down, or store RNA according to your typical protocol.
Option 2 - Following application to RNArchive dry RNA and store or transport at ambient temperature. Following rehydration, RNArchive conveys additional stability to RNA in liquid form for up to 8 hours at room temperature (21-25°C) or on ice. Following the 8 hour post-recovery period, dry your sample down again for storage or store
according to your typical protocol.
Yes, RNA in RNArchive can be rehydrated and re-dried up to five times.
Yes, the RNArchive matrix protects RNA in the liquid state at room temperature during the drying process. This protection extends for up to 48 hours.
After addition of molecular-grade water, your samples will be in the same buffer they were stored in at the time of application.
Following recovery, RNA may be stored for up to 8 hours at room temperature (21-25°C), and then dry down or store according to your typical protocol.
Additional purification is not required prior to performing downstream applications. Similar RNA quality is maintained before and after recovery.
RNArchive is not a filter, beads, silica column or paper. RNArchive is a water soluble, chemical matrix
Yes, the RNase inhibitor in RNArchive is an inhibitor of DNase I. However, because of the low concentration of inhibitor it should be easy to swamp the inhibition by simply increasing the amount of DNase I used.
No, the chemical matrix used to store DNA is not the same as the chemical matrix used to store RNA.
Yes, but special care and handling is recommended. When applying the sample be especially careful to place it in the very bottom of the tube. When recovering the sample we recommend using a minimum of twice the original volume to ensure you rehydrate all the sample. Refer to our tutorial on handling small sample volumes for further details.
At its normal concentration range there is no effect on the polymerase reaction and we have done many library preps on RNArchive protected samples with no problems at all. Furthermore, as the sample moves thought the library construction the RNArchive is quickly diluted away.
Yes, unless the RNArchive is diluted by >10-100 times compared to its normal concentration it will be a potent inhibitor of the RNase used to remove the starting RNA.