RNAssure
Room temperature stabilization and protection for purified RNA
AMSBIO's RNAssure stops RNA degradation with patented Active Chemical Protection™ (ACP).
• Hassle-free integration with your chosen purification method
• Handle RNA for 3 days without freezing
• Maintain the integrity of your samples for downstream processes
• Use with both phenol and column-based methods
Features
With RNAssure, you elute RNA from your purification column to be protected straight away
• RNA suitable for RNAseq
• Protects RNA from freezer failure.
• Rapid RNase inactivation
• Allows RNA handling without loss of integrity
• Prevents contamination from ice bath water.
Applications
RNAssure facilitates workflows such as:
• Phenol-based purification integration
• Column-based purification integration
• Downstream RNAseq
• Downstream PCR investigations
RNAssure is compatible with all major workflows
RNAssure Elution Tubes replace those of standard purification kits to efficiently collect purified RNA as it is eluted from your chosen purification column.
RNA stabilised by RNAssure does not require additional clean up steps to be utilised downstream applications like quantitation, gel or Bioanalyzer™ analysis, RT-PCR, and NGS library preparation. The patented Active Chemical Protection™ technology works to inactivate all nucleases, protect against oxidation and prevent adherence of RNA to plastic surfaces.
Storage Condition | Length of Protection |
---|---|
Room temperature (15-25°C) | 3 days |
Refrigeration (2-8°C) | 2 weeks |
Frozen (<-70°C) | >1 year |
Highly stable RNA at room temperature
RNA is a delicate molecule to work with. While on the bench, sample temperature can often rise above 0°C. Even for short periods this can be harmful to investigations. While the freezing of samples does slow done the actions of RNAses, oxidation and hydrolysis, our Active Chemical Protection™(ACP) works to stop these degrading factors to ensure the highest quality nucleic acids at room temperature.
At room temperature without RNAssure, samples show statistically significant degradation after just 15 minutes. Whereas, samples with RNAssure treatment, show no signs of degredation after 4 hours.
RNAssure Frequently Asked Questions
RNAssure is a water soluble, chemical matrix provided dried in a green microcentrifuge fliptop tube. RNAssure is not composed of any filters, beads, or paper.
Yes, additional purification is not required prior to performing downstream applications. The
RNAssure does not interfere with any downstream protocol.
No, the inhibitor concentration used in RNAssure will not interfere with common DNase treatment protocol.
Collecting less than 20 μL will increase the concentration of the active ingredient which may inhibit downstream analysis.
Yes, RNAssure Elution Tube can be used without the need for manufacturer kits with spin columns. After the final elution or resuspension step, collect 20 – 150 μL of the RNA sample and add it to the RNAssure Elution tube. Mix by gently tapping for 10-15 seconds or pipetting up and down 10 times to solubilize and mix the RNAssure stabilization agent into the purified RNA sample
Yes, RNAssure Elution Tubes are compatible with whole blood stabilization tubes.
No, at its normal concentration there is no effect on the reverse transcriptase or polymerase reactions.
RNAssure contains potent inhibitors of all RNases. To ensure proper removal of RNA templates, ensure that the input RNAssure stabilized RNA sample constitutes less than 10% of the total reaction volume.
There is no limitation of RNA concentration. However, follow the RNA extraction kit manufacturer’s recommendation and do not exceed the recommended total RNA amount
Yes, RNAssure is a chemical matrix that effectively protects all forms of RNA.
RNAssure will not affect 260/280 ratio. However, it has an absorbance of approximately 0.4 OD at 230 nm (e.g. NanoDrop analysis) and will cause a slightly higher 230 nm sample reading and therefore reduce the 260/230 ratio value. If desired, a “blank” or control sample can be made. To do this, add same volume of nuclease-free water to an empty RNAssure tube.