Skeletal Muscle

AMSBIO's guide to source, grow and differentiate human skeletal muscle cells

Skeletal muscle is one of the most important tissues in the human body, accounting for approximately 30-40% of the weight in a healthy individual, and vital for voluntary movement. During development skeletal muscle grows in cell number and size. In adult tissue, function and size is maintained through regeneration after muscle injury by skeletal muscle progenitor cells (also known as satellite cells). Therefore, impairments in muscle regeneration can cause deterioration in muscle function. Modelling human skeletal muscle differentiation is important for understanding muscle function and for preclinical investigations into muscle-related diseases and drug discovery.

Muscle differentiation

Skeletal muscle regeneration relies on adult stem cells called satellite cells. These satellite cells are quiescent but can respond to injury or growth signals to regenerate tissue through re-entering the cell cycle. Activated satellite cells first proliferate and differentiate into myoblasts, which can then further differentiate into myocytes and fuse to form multi-nucleated myotubes. Myotubes then bundle together to form mature contractile myofibers.

Alternatively, pluripotent stem cells (ESCs and iPSCs) have been used as a source for skeletal muscle cells through directed differentiation providing a valuable tool for tissue engineering approaches.

AMSBIO's workflow

At AMSBIO we can streamline Skeletal muscle modelling by offering a supply of different skeletal muscle cell sources to suit your needs, differentiation kits for iPSCs and compatible media in order to achieve muscle cell maturity. Our media and reagents provide a physiologically relevant cell culture system to resemble the in vivo environment for disease modelling and drug discovery applications.

Skeletal Muscle Cell Lines

We offer a range of skeletal muscle cell lines cryopreserved and ready-to-use.

Visit our biorepository range to select your source cell line or tissue of interest.

Name	Datasheet	Packsize	Order
Cryopreserved\|Human Skeletal Muscle (500\|000 cells/vial) Lean Donor\|BMI<25.0	-	each	View
Cryopreserved\|Human Skeletal Myoblast (500\|000 cells/vial)-DIABETIC DONOR	-	each	View
Cryopreserved\|Human Skeletal Myoblasts (500\|000 cells/vial)		each	View
Cryopreserved\|Human Skeletal Myoblasts (500\|000 cells/vial)\|POOLED Donor lot		each	View
Human Primary Skeletal Muscle Cells	-	Each vial contains >5 x 10^5 cells in 1ml volume	View

Skeletal Muscle Differentiation

Skeletal Muscle Differentiation Kit

Differentiate human pluripotent stem cells using a simple 3-step process of media change and cell passaging. The kit achieves typically 70% MF20-positive myotubes without the need for cell sorting or transfection. Options are also available for differentiating skeletal muscle progenitors to mature myotubes.

Quick-Muscle™ Skeletal - SeV Kit

Differentiate your human iPS/ES cells into skeletal muscle cells in just 7 days producing highly pure populations of skeletal muscle cells using a temperature-sensitive Sendai virus.

The skeletal muscle cells produced display typical skeletal muscle morphology and markers, such as myosin heavy chain (MHC). Following day 7, the cells may be maintained in the maintenance medium best suited to your needs (see below for our range of muscle maintenance medium).

Name	Datasheet	Packsize	Order
Quick-Muscle™ Skeletal - SeV Kit (Small)	-	4 wells of a 24-well plate (Small)	View
Skeletal Muscle. Differentiation Media Kit, 4x250mL (+ 1 x Myoblast Vial)*		4 x 250 ml + 1 vial	View
Skeletal Muscle. Differentiation Media Kit, 4x250mL		4x250mL	View

Induction and Maturation Media

For Skeletal Muscle culture we offer maturation media for different stages of differentiation in order to reach your target population of cells.

Name	Datasheet	Packsize	Order
Culture medium for maturation of muscle progenitor cells (100 mL) with supplements (100 µL)	-	101 mL + 100 µL	View
Culture medium for maturation of muscle progenitor cells (500 mL) with supplements (5x100 µL)	-	501 mL + 5x100 µL	View
Quick-Muscle™ Skeletal - Maintenance Medium	-	4 wells of a 24-well plate for 2 weeks	View
Skeletal Muscle Cell Growth Medium\| 500ml		500ml	View
Skeletal Muscle. Induction Medium, 250mL Bottle		250ml	View
Skeletal Muscle. Myoblast Medium, 250mL Bottle		250ml	View
Skeletal Muscle. Myotube Fusion Medium, 250mL Bottle		250ml	View
Skeletal Muscle. Myotube Medium, 250mL Bottle		250ml	View

Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications.

Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications.

Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.

We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.

We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.

We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.

We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.

We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.

Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.

Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.

Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.

Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.

Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.

Skeletal Muscle

AMSBIO's guide to source, grow and differentiate human skeletal muscle cells

Muscle differentiation

AMSBIO's workflow

Skeletal Muscle Cell Lines

Skeletal Muscle Differentiation

Skeletal Muscle Differentiation Kit

Quick-Muscle™ Skeletal - SeV Kit

Induction and Maturation Media

Analysis

Antibodies

Assays and Kits

Related Product Info Pages

CELLBANKER® Cell Freezing Media

iMatrix-511 Recombinant Laminin E8 Fragments

iPSC-Derived Cells and Differentiation Kits

Skeletal Muscle Differentiation Kit

StemFit® Feeder-Free Stem Cell Culture Media

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