Our Services
Stable Cell Line Construction
You choose a constitutive or inducible promoter, a tag, a selection marker and a fluorescent marker. Either you provide target templates or we design, synthesize or obtain it from a cDNA collection. We will then:
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- Clone your ORF or shRNA into our lentiviral vector
- Generate lentivirus
- Transduce the cell line of interest
- Select the stably transduced, high expression cells
- Validate the genomic integration of the target by genomic PCR
- Validate the high-expression clone by Western Blot (if applicable)
- Produce two cryogenic preserved vials of stable cells and a final report
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Why use AMSBIO's stable cell line services?
- Safe-to-use (self-inactivating) lentiviral particles can deliver your gene into a wide range of cell lines including non-dividing, primary or stem cells
- Engineered in-house lentiviral vector for highly efficient gene integration into cell genome
- You can choose to have an inducible or constitutive promoter
- Our experts have years of experience in lentiviral cloning and expression
- Fast turnaround time
- The best price and the best quality in its class
Stable Cell Lines Service Request
![molecular_biology](https://www.amsbio.com/wp//wp-content/uploads/2019/10/molecular_biology.jpg)
Let's get started
Complete our service request form and we will contact you to discuss your requirements.