TUNR Flexible Gene Editing System
TUNR is a new gene editing technology that works with CRISPR-Cas9 to allow for controlled gene knockdown. AMSBIO’s TUNR kits leverage the CRISPR-Cas9 system for the targeted integration of a polyA track at a precise location within a targeted gene, resulting in reduced gene expression.
Our kits allow you to precisely tune gene expression from 100% all the way down to complete knockout, model ranges of gene expression reflecting diversity in patient populations and knockdown essential genes where knockout would result in lethality. In addition, a kit is available that allows you to control endogenous gene expression enabling elimination of artifacts from ectopic expression.
Our kits are custom-designed for your project and include everything you need to fine-tune gene expression in your favorite cell lines. Just contact us to discuss your requirements.
Benefits
- Generates predictable levels of knockdown
- Knockdown is permanent and heritable
- Few off-target effects
- TUNR expression is intrinsically linked with gene target expression
Validated In
- Human cells
- Plants
- Bacteria
- Yeast
- Protozoans
- Insects
Types of Custom Kits
Endogenous Genes
The TUNR Flexible Gene Editing System for Endogenous Genes allows you to generate four cell lines or animal models with four different levels of knockdown of your endogenous gene of interest.
Targeted Transgenes
The TUNR Targeted Transgenic Kit enables you to insert your gene of interest under TUNR control into the AAVS1 locus. AAVS1 is a “safe harbor”—a safe place to integrate transgenes, allowing predictable expression from a locus known not to alter the expression of other genes or have otherwise deleterious effects on the cell.
Plasmid-Delivered Transgenes
The TUNR Plasmid-Delivered Transgenes Kit allows you to control the expression of a transgene. Simply transfect your cells with one of our four plasmids containing your gene under the control of TUNRs of different strengths.
How does the TUNR Flexible Gene Editing System work?
